Polyclonal Antibody to Sclerostin (SOST) Rattus norvegicus (Rat) Polyclonal antibody

VBCH; Sclerosteosis

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC864Ra01-Recombinant Sclerostin (SOST)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Sclerostin (SOST) Packages (Simulation)
  • Polyclonal Antibody to Sclerostin (SOST) Packages (Simulation)
  • Polyclonal Antibody to Sclerostin (SOST) Western Blot; Sample: Hepa1-6 cell lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Rat SOST Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SOST. It has been selected for its ability to recognize SOST in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in OsteocytesPubMed: 23357248
  • Acute effects of glucocorticoids on serum markers of osteoclasts, osteoblasts, and osteocytesPubmed: 23247536
  • Serum Sclerostin in Adult High-Activity Patients with Juvenile Idiopathic ArthritisPubmed:Pmc4236454
  • Early effects of tumor necrosis factor inhibition on bone homeostasis after soluble tumor necrosis factor receptor usePubmed:Pmc4219971
  • The Anti-Osteoanabolic Function of Sclerostin is Blunted in Mice Carrying a High Bone Mass Mutation of Lrp5Pubmed:25640331
  • DMP-1-mediatedPubMed: 26481310
  • Effect of Mirtazapine on Rat Bone Tissue after OrchidectomyPubMed: 25871861
  • The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolismPubmed:29428468
  • EFFECTS OF DISEASE-MODIFYING ANTIRHEUMATIC DRUG TREATMENT ON PERIARTICULAR BONE REMODELING IN RHEUMATOID AND PSORIATIC …:
  • Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism
  • Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in …Pubmed: 33065276
  • Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats34373766

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