Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B)

CD120B; P75; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR2; TNFR80; P75tnfr; Tumor necrosis factor receptor 2; p80 TNF-alpha receptor; Etanercept
Western Blot

IMMUNOGEN INFORMATIONImmunogen: Recombinant TNFRSF1B (Ala25~Met229) expressed in E.coli.
Accession No.: RPB504Ra01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AKVVLT PYKPEPGNQC QISQEYYDKK AQMCCAKCPP GQYAKHFCNK TSDTVCADCA AGMFTQVWNH LHTCLSCSSS CSDDQVETHN CTKKQNRVCA CNADSYCALK LHSGNCRQCM KLSKCGPGFG VARSRTSNGN VICSACAPGT FSDTTSSTDV CRPHRICSIL AIPGNASTDA VCASESPTPS AVPRTIYVSQ PEPTRSQPM
ANTIBODY SPECIFITY The antibody is a rabbit polyclonal antibody raised against TNFRSF1B. It has been selected for its ability to recognize TNFRSF1B in immunohistochemical staining and western blotting. APPLICATIONS WB, ICC, IHC-P, IHC-F, ELISA
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. QUALITY CONTROL Content: The quality control contains recombinant TNFRSF1B (Ala25~Met229) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Leave your messages here

Welcome to our website. If you have any questions, please send a message to us.
We'll Feedback to you within 24 hours.Our work time is from AM 8:00 to PM 4:30 (U.S. Central Time)

  • Sales:sales@cloud-clone.us
  • After-sales:service@cloud-clone.us
  • Email:mail@cloud-clone.us
  • Close
    Polyclonal Antibody to Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Material Safety Data Sheet International Organization for Standardization guarantee