Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) Mus musculus (Mouse) Polyclonal antibody

SLC25A8; UCPH; Solute carrier family 25 member 8 ;Uncoupling Protein 2, Mitochondrial

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC586Mu01-Recombinant Mitochondrial Uncoupling Protein 2 (UCP2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) Packages (Simulation)
  • Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) Packages (Simulation)
  • Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) DAB staining on IHC-P; Samples: Mouse Lung Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse UCP2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) DAB staining on IHC-P;
    Samples: Mouse Liver Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse UCP2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) DAB staining on IHC-P;
    Sample: Mouse Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse UCP2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC586Mu01.jpg Figure. Western Blot; Sample: Recombinant UCP2, Mouse.
  • Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) Western Blot; Sample: Lane1: Mouse Skeletal muscle lysate; Lane2: Rat Skeletal muscle lysate; Lane3: Mouse Heart lysate; Lane4: Rat Heart lysate; Lane5: Mouse Kidney lysate; Lane6: Mouse Lung lysate; Lane7: Rat Lung lysate; Lane8: Mouse Spleen lysate; Lane9: Mouse Thymus lysate; Lane10: Rat Thymus lysate
    Primary Ab: 1μg/ml Rabbit Anti-Mouse UCP2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mitochondrial Uncoupling Protein 2 (UCP2) Western Blot; Sample: Lane1: Mouse Spleen lysate; Lane2: Human Jurkat cell lysate
    Primary Ab: 2µg/ml Rabbit Anti-Mouse UCP2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against UCP2. It has been selected for its ability to recognize UCP2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • FM19G11 Favors Spinal Cord Injury Regeneration and Stem Cell Self-Renewal by Mitochondrial Uncoupling and Glucose Metabolism InductionPubMed: 22865656
  • A 4-week consumption of medium roast and dark roast coffees affects parameters of energy status in healthy subjectsSciencedirect:S0963996914002403
  • The effect of delivery type on uncoupling protein-2 levelsPubMed: 26479203
  • Vitamin C and E chronic supplementation differentially affect hepatic insulin signaling in ratsPubmed:29291420
  • Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosisPubmed: 32911041
  • Vitamin B12 ameliorate Tramadol-induced oxidative stress, endocrine imbalance, apoptosis and NO/iNOS/NF-κB expression in Sprague Dawley through regulatory …34923198

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