Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP) Homo sapiens (Human) Polyclonal antibody

ADAMTS13; TTP; vWF-cp; vWF-cleaving protease; A Disintegrin And Metalloproteinase With A Thrombospondin Type 1 Motif Member 13

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA950Hu01-Recombinant Von Willebrand Factor Cleaving Protease (vWFCP)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP) Packages (Simulation)
  • Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP) Packages (Simulation)
  • Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP) Used in DAB staining on fromalin fixed paraffin- embedded liver tissue
  • Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP) AF488 staining on IF;
    Sample: MCF7 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human vWFCP Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA950Hu01.jpg Figure. Western Blot; Sample: Recombinant vWFCP, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against vWFCP. It has been selected for its ability to recognize vWFCP in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Neutrophil Protease Cleavage of Von Willebrand Factor in Glomeruli - An Anti-thrombotic Mechanism in the Kidney.pubmed:28139439
  • Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …
  • Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep ApneaPubmed: 31882827

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