KO-Validated
Polyclonal Antibody to X-linked Inhibitor Of Apoptosis Protein (XIAP)

BIRC4; API3; ILP1; MIHA; XLP2; Baculoviral IAP Repeat-Containing 4; E3 ubiquitin-protein ligase XIAP; IAP-like; Inhibitor of apoptosis protein 3; Inhibitor Of Apoptosis Protein, X-Linked

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAC606Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryApoptosis

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC606Hu01-Recombinant X-linked Inhibitor Of Apoptosis Protein (XIAP)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 30ug/ml Rabbit Anti-Human XIAP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human XIAP Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human XIAP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human XIAP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human HepG2 Cells;
Primary Ab: 20µg/ml Rabbit Anti-Human XIAP Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human XIAP Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;Sample: MCF7 cellPrimary Ab: 20µg/ml Rabbit Anti-Human XIAP AntibodySecond Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant XIAP, Human.
Western Blot; Sample: Lane1: HepG2 cell lysate; Lane2: 293T cell lysate; Lane3: Jurkat cell lysate; Lane4: Hela cell lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Heart lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human XIAP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: XIAP knockout 293T cell lysate;
Predicted MW: 56kd
Observed MW: 55kd
Primary Ab: 1µg/ml Rabbit Anti-Human XIAP Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against XIAP. It has been selected for its ability to recognize XIAP in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 2-30µg/mL;
Immunocytochemistry: 2-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)