Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3) Pan-species (General) Labelled antibody

Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol

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Overview
Properties
  • SourceAntibody labeling
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelBiotin
  • Original Antibody n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3) Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against VD3. It has been selected for its ability to recognize VD3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Ratsprofile:303311214
  • Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats:
  • Deficiencia de vitamina D en madres y neonatos mexicanospubmed:29099117
  • P38/TRHr-Dependent Regulation of TPO in Thyroid Cells Contributes to the Hypothyroidism of Triclosan-Treated RatsPubmed:29462796

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