Monoclonal Antibody to Beclin 1 (BECN1) Homo sapiens (Human) Monoclonal antibody

ATG6; VPS30; Coiled-Coil,Myosin-Like BCL2 Interacting Protein; ATG6 Autophagy Related 6 Homolog

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C9
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPJ557Hu01-Recombinant Beclin 1 (BECN1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Beclin 1 (BECN1) Packages (Simulation)
  • Monoclonal Antibody to Beclin 1 (BECN1) Packages (Simulation)
  • MAJ557Hu23.jpg Western Blot; Sample: Lane1: T-47D cell lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate Primary Ab: 5μg/ml Mouse Anti-Human BECN1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against BECN1. It has been selected for its ability to recognize BECN1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-5µg/mL
Immunohistochemistry: 5-50µg/mL
Immunocytochemistry: 5-50µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Can Enhanced Autophagy be Associated with Human Longevity? Serum Levels of the Autophagy Biomarker Beclin-1 are Increased in Healthy CentenariansPubmed:25307612
  • A peel-off facial mask comprising myoinositol and trehalose-loaded liposomes improves adult female acne by reducing local hyperandrogenism and activating autophagypubmed:28342238
  • The novel CXCR4 antagonist, PRX177561, reduces tumor cell proliferation and accelerates cancer stem cell differentiation in glioblastoma preclinical modelspubmed:28639900
  • Beclin1 circulating levels and accelerated ageing markers in COPD DOI: 10.1183/1393003.congress-2017.PA1018
  • Beclin1 circulating levels and accelerated aging markers in COPDPubmed:29402890
  • A novel combined score of biomarkers in sputum may be an indicator for lung cancer: A pilot studyDoi: 10.1016/j.cca.2018.09.027
  • Serum concentration of autophagy-related protein 7 is correlated with non-alcoholic fatty liver disease in Chinese adults
  • Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study33936265
  • Inhibition of HepG-2 Cells (Liver Cancer Cell Line) Viability by 3-Hydroxypyridine-2-Carboxaldehyde N (4)-Methylthiosemicarbazone
  • BIOMARKERS OF AUTOPHAGY AND ANTIVIRAL ROLE OF PLACENTA FOR PREDICTING PRETERM DELIVERY IN PATIENTS WITH PRETERM DELIVERY?¡­
  • IDENTIFICATION OF FACTORS CONNECTED WITH AUTOPHAGY ACTIVITY UNDER CONDITIONS OF PARTIAL NUTRITIONAL DEPRIVATION IN MEN

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