Monoclonal Antibody to Cytokeratin 7 (CK7) Homo sapiens (Human) Monoclonal antibody

KRT7; K2C7; K7; SCL; Keratin 7; Sarcolectin; Keratin,55K Type II Cytoskeletal; Keratin, type II cytoskeletal 7

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number C5
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Cytokeratin 7 (CK7) Packages (Simulation)
  • Monoclonal Antibody to Cytokeratin 7 (CK7) Packages (Simulation)
  • Monoclonal Antibody to Cytokeratin 7 (CK7) Western Blot; Sample: Lane1: Human Saliva; Lane2: Human Lung lysate; Lane3: A549 cell lysate; Lane4: Hela cell lysate; Lane5: HepG2 cell lysate
    Primary Ab: 0.2µg/ml Mouse Anti-Human CK7 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CK7. It has been selected for its ability to recognize CK7 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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