Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15) Rattus norvegicus (Rat) Monoclonal antibody

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number D3
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPL154Ra01-Recombinant Fibroblast Growth Factor 15 (FGF15)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15) Packages (Simulation)
  • Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15) Packages (Simulation)
  • Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15) DAB staining on IHC-P; Samples: Rat Liver Tissue; Primary Ab: 10µg/ml Mouse Anti-Rat FGF15 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15) DAB staining on IHC-P;
    Samples: Rat Testis Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Rat FGF15 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Fibroblast Growth Factor 15 (FGF15) DAB staining on IHC-P;
    Samples: Rat Kidney Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Rat FGF15 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against FGF15. It has been selected for its ability to recognize FGF15 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21PubMed: 25774553
  • Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammationpubmed:27580383
  • Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis.pubmed:28130274
  • The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance10.1186/s12902-017-0211-5
  • The ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in micePubmed:29468415
  • FGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver TumorigenesisPubmed: 30745141
  • Interactions between the gravitostat and the fibroblast growth factor system for the regulation of body weight
  • Lactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axisPubmed: 31626262
  • Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activationPubmed: 33036625
  • Si-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis34736501
  • Dynamics of the gut-liver axis in rats with varying fibrosis severityPubmed:35637968

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