Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) Homo sapiens (Human) Monoclonal antibody

HDAC1; GON10; HD1; RPD3; RPD3L1

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number E20
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) Packages (Simulation)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) Packages (Simulation)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Human Placenta Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human HDAC1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Porcine Cerebrum Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human HDAC1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Porcine Uterus Tissue
    Primary Ab: 20µg/ml Mouse Anti-Human HDAC1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Rat Cerebellum Tissue
    Primary Ab: 5µg/ml Mouse Anti-Human HDAC1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Histone Deacetylase 1 (HDAC1) DAB staining on IHC-P;
    Sample: Rat Kidney Tissue
    Primary Ab: 5µg/ml Mouse Anti-Human HDAC1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against HDAC1. It has been selected for its ability to recognize HDAC1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylasePubmed:25609369
  • Effects of tetrandrine and caffeine on cell viability and expression of mammalian target of rapamycin, phosphatase and tensin homolog, histone deacetylase 1, and histone acetyltransferase in glioma cellsScience: Article
  • Uridine protects against hypoxic-ischemic brain injury by reducing histone deacetylase activity in neonatal ratsPubMed: 26410212
  • Lacosamide reduces HDAC levels in the brain and improves memory: Potential for treatment of Alzheimer's diseasePubMed: 25931268
  • Effects of caffeine on cell viability and activity of histone deacetylase 1 and histone acetyltransferase in glioma cellsS1016319016300295

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