Monoclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM) Homo sapiens (Human) Monoclonal antibody

CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number C4
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM) Packages (Simulation)
  • Monoclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM) Packages (Simulation)
  • Monoclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM) Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HUVEC cell lysate;
    Primary Ab: 0.2µg/ml Mouse Anti-Human HCAM Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM) Human peripheral blood lymphocytes were stained with 10µg/ml mouse Anti-human HCAM Monoclonal Antibody (Catalog MAA670Hu23, green histogram) or Isotype control antibody (Catalog IS067-Mu01, blue histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against HCAM. It has been selected for its ability to recognize HCAM in immunohistochemical staining and western blotting.

Usage

Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Predictive Value of Soluble PD-1, PD-L1, VEGFA, CD40 Ligand and CD44 for Nivolumab Therapy in Advanced Non-Small Cell Lung Cancer: A Case-Control StudyPubmed: 32085544

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