Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) Homo sapiens (Human) Monoclonal antibody

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number D7
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA085Hu01-Recombinant Leukemia Inhibitory Factor (LIF)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) Packages (Simulation)
  • Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) Packages (Simulation)
  • Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 20µg/ml Mouse Anti-Human LIF Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Leukemia Inhibitory Factor (LIF) DAB staining on IHC-P;
    Sample: Human Skeletal muscle Tissue;
    Primary Ab: 20µg/ml Mouse Anti-Human LIF Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against LIF. It has been selected for its ability to recognize LIF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioidspubmed:28077540
  • A study of altered cytokine rhythms associated with successful implantation in cows10.1080/09291016.2017.1361159
  • Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database

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