Monoclonal Antibody to Semaphorin 3A (SEMA3A) Homo sapiens (Human) Monoclonal antibody

SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a Kappa, Clone Number C2
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPL917Hu01-Recombinant Semaphorin 3A (SEMA3A)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Semaphorin 3A (SEMA3A) Packages (Simulation)
  • Monoclonal Antibody to Semaphorin 3A (SEMA3A) Packages (Simulation)
  • Monoclonal Antibody to Semaphorin 3A (SEMA3A) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10µg/ml Mouse Anti-Human SEMA3A Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Semaphorin 3A (SEMA3A) DAB staining on IHC-P;
    Samples: Human Heart Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human SEMA3A Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Semaphorin 3A (SEMA3A) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human SEMA3A Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Semaphorin 3A (SEMA3A) DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human SEMA3A Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAL917Hu21.jpg Figure. Western Blot; Sample: Recombinant SEMA3A, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against SEMA3A. It has been selected for its ability to recognize SEMA3A in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosusBiomed: ar3881
  • Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to PruritusPubMed: 26121211
  • Neurofilament light chain level is a weak risk factor for the development of MS.pubmed:27521440
  • miR-126-5p promotes retinal endothelial cell survival through SetD5 regulatio in neuronspubmed:29180574
  • A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells
  • Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway.Pubmed: 32603817
  • Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 SignalingPubmed: 32413258
  • Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes33905439

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