Monoclonal Antibody to Thioredoxin (Trx) Rattus norvegicus (Rat) Monoclonal antibody

TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Thioredoxin (Trx) Packages (Simulation)
  • Monoclonal Antibody to Thioredoxin (Trx) Packages (Simulation)
  • Monoclonal Antibody to Thioredoxin (Trx) AF488 staining on IF;
    Sample: HepG2 cell
    Primary Ab: 30µg/ml Mouse Anti-Rat Trx Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against Trx. It has been selected for its ability to recognize Trx in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis OxfordJournals: source
  • Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosusPubmed:Pmc4061188
  • Alteration in the intrafollicular thiol–redox system in infertile women with endometriosisPubmed:25376627
  • Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stressPubmed:25564095
  • Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung.Pubmed:26797643
  • Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the …Pubmed: 30351992
  • Redox, immune and genetic biomarker system for personalized treatments in colorectal cancerDoi: 10.4251/wjgo.v11.i2.117
  • The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in TriathletesPubmed: 30736479
  • Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitusPubmed: 31351037
  • Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
  • Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ responsePubmed: 33161477
  • Examination of androgenetic alopecia with serum biomarkersPubmed: 32969583
  • Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats34159266
  • Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat34155936

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