Monoclonal Antibody to Transglutaminase 2 (TGM2) Mus musculus (Mouse) Monoclonal antibody

tTG; TG2; C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase 2, Tissue; Transglutaminase C; Transglutaminase H

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C2
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB830Mu01-Recombinant Transglutaminase 2 (TGM2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Transglutaminase 2 (TGM2) Packages (Simulation)
  • Monoclonal Antibody to Transglutaminase 2 (TGM2) Packages (Simulation)
  • Monoclonal Antibody to Transglutaminase 2 (TGM2) FITC staining on IF; Sample: Human Hela cell; Primary Ab: 30µg/ml Mouse Anti-Mouse TGM2 Antibody Second Ab: 5µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against TGM2. It has been selected for its ability to recognize TGM2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Tissue transglutaminase is involved in mechanical load–induced osteogenic differentiation of human ligamentum flavum cellsPubmed:27115725
  • ECM Crosslinking Enhances Fibroblast Growth and Protects Against Matrix Proteolysis in Lung Fibrosis.pubmed:29053339
  • Metformin attenuates TGF‑β1‑induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF‑β pathwaysPubmed: 32550106

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