Monoclonal Antibody to Tumor Protein p53 (P53) Rattus norvegicus (Rat) Monoclonal antibody

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a lambda, Clone Number D10
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA928Ra01-Recombinant Tumor Protein p53 (P53)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.45mg/ml
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  • Monoclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Monoclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Monoclonal Antibody to Tumor Protein p53 (P53) FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 40μg/ml Mouse Anti-Rat TP53 Antibody Second Ab: 5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
  • MAA928Ra22.jpg Western Blot; Sample: Lane1: Rat Colon lysate; Lane2: A431 cell lysate Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Lane1: Porcine Colon lysate; Lane2: Porcine Spleen lysate; Lane3: Porcine Mammary gland lysate; Lane4: Porcine Skin lysate
    Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: RAW264.7 cell lysate; Lane3: Canine Colon lysate; Lane4: Gallus Small intestine lysate; Lane5: Bovine Colon lysate; Lane6: Caprine Colon lysate
    Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-40µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Chicken (Gallus)

Giveaways

Citations

  • Analysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Therapeutic role of curcumin in oxidative DNA damage caused by formaldehydePubMed: 25761397
  • Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing micePubMed: 26344777
  • Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytesPubmed: 31331641
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …

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