KO-Validated
Polyclonal Antibody to Canopy 2 Homolog (CNPY2)

ZSIG9; Cnpy2; MSAP; TMEM4; Transmembrane Protein 4; MIR-interacting saposin-like protein; Putative secreted protein Zsig9

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 116.00 US$ 270.00 US$ 386.00 US$ 965.00 US$ 3,860.00
Quantity

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Overview

Properties

Product No.PAM449Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPM449Hu01-Recombinant Canopy 2 Homolog (CNPY2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CNPY2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;Sample: MCF7 cellPrimary Ab: 20µg/ml Rabbit Anti-Human CNPY2 AntibodySecond Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant CNPY2, Human.
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: CNPY2 knockout MCF7 cell lysate;
Predicted MW: 21,9kd
Observed MW: 20kd
Primary Ab: 1µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Pancreas lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Sample: MCF7 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Sample: HepG2 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
Western Blot; Sample: Lane1: HepG2 cell lysate; Lane2: Hela cell lysate; Lane3: Mouse Pancreas lysate; Lane4: Porcine Liver lysate; Lane5: Rat Liver lysate
Primary Ab: 1µg/ml Rabbit Anti-Human CNPY2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CNPY2. It has been selected for its ability to recognize CNPY2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;1:470-1900
Immunohistochemistry: 5-20µg/mL;1:47-190
Immunocytochemistry: 5-20µg/mL;1:47-190
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)