Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) Mus musculus (Mouse) Polyclonal antibody

GSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC992Mu01-Recombinant Glutathione Peroxidase 3, Plasma (GPX3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) Packages (Simulation)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) Packages (Simulation)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) DAB staining on IHC-P;
    Samples: Mouse Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) DAB staining on IHC-P;
    Samples: Mouse Stomach Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) DAB staining on IHC-P;
    Samples: Mouse Heart Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) DAB staining on IHC-P;
    Samples: Mouse Cerebrum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) DAB staining on IHC-P;
    Samples: Mouse Uterus Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX3 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC992Mu01.jpg Figure. Western Blot; Sample: Recombinant GPX3, Mouse.
  • Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3) Western Blot; Sample: Rat Kidney lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Mouse GPX3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GPX3. It has been selected for its ability to recognize GPX3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Alteration in the intrafollicular thiol–redox system in infertile women with endometriosisPubmed:25376627
  • Endothelial function impairment in STEMI patients with out-of-hospital cardiac arrest under therapeutic hypothermia treatment.pubmed:28087172
  • Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients.pubmed:27748187
  • Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress.pubmed:28702016
  • Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1Pubmed: 30284230
  • Kidney toxicity and response of selenium containing protein-glutathione peroxidase (Gpx3) to CdTe QDs on different levelsPubmed: 30535317
  • Human Plasma Protein (s) Based Conceptual Diagnostic Tool for Altitude-Acclimation Assessment
  • Dietary selenium deficiency or selenomethionine excess drastically alters organ selenium contents without altering the expression of most selenoproteins in mice
  • Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P
  • In vivo and in vitro studies on inactivation of selenium containing protein-glutathione peroxidase 3 in mice nephrocytes caused by leadPubmed: 32678766
  • Selenium–GPX4 axis protects follicular helper T cells from ferroptosis34413521
  • Experimental nephrotic syndrome in mice with an inducible deletion of podocin and the role of plasminogen in sodium retention

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