Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) Rattus norvegicus (Rat) Polyclonal antibody

GST-A4; GSTA4-4; GTA4

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA659Ra01-Recombinant Glutathione S Transferase A4 (GSTA4)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) Packages (Simulation)
  • Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) Packages (Simulation)
  • Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) DAB staining on IHC-P; Samples: Rat Colon Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat GSTa4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) DAB staining on IHC-P;
    Sample: Rat Testis Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat GSTa4 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) DAB staining on IHC-P;
    Sample: Rat Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat GSTa4 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) DAB staining on IHC-P;
    Sample: Rat Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat GSTa4 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
    Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA659Ra01.jpg Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Lung lysate Primary Ab: 0.2µg/ml Rabbit Anti-Rat GSTA4 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
  • Polyclonal Antibody to Glutathione S Transferase A4 (GSTA4) Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Liver lysate
    Primary Ab: 5µg/ml Rabbit Anti-Rat GSTA4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19) Selected
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GSTA4. It has been selected for its ability to recognize GSTA4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Glutathione S-transferase alpha 4 induction by activator protein 1 in colorectal cancerPubmed:27065323

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