Polyclonal Antibody to Histone Deacetylase 4 (HDAC4)

HD4; HDAC-A; HDACA

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 108.00 US$ 252.00 US$ 360.00 US$ 900.00 US$ 3,600.00
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Overview

Properties

Product No.PAE904Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE904Mu01-Recombinant Histone Deacetylase 4 (HDAC4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.84mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse HDAC4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue

Figure. Western Blot; Sample: Recombinant HDAC4, Mouse.
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse HDAC4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse HDAC4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Mouse HDAC4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-Mouse HDAC4 Polyclonal Antibody (Catalog PAE904Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HDAC4. It has been selected for its ability to recognize HDAC4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)