Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2) Homo sapiens (Human) Polyclonal antibody

HDMX; hdm2; Mouse Double Minute 2,Human Homolog Of p53-Binding Protein; Oncoprotein Mdm2

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPG790Hu01-Recombinant Mdm2 p53 Binding Protein Homolog (MDM2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2) Packages (Simulation)
  • Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2) Packages (Simulation)
  • Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2) IHC Images
  • PAG790Hu01.jpg Western Blot; Sample: Mouse Lung lysate Primary Ab: 0.2µg/ml Rabbit Anti-Human MDM2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2) Western Blot; Sample: Lane1: Mouse Cerebellum lysate; Lane2: Human MCF7 cell lysate;
    Primary Ab: 2µg/mL Rabbit Anti-Human MDM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2) Western Blot; Sample: U2OS cell lysate
    Primary Ab: 0.2μg/ml Rabbit Anti-Human MDM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mdm2 p53 Binding Protein Homolog (MDM2) Western Blot; Sample: Lane1: U2OS cell lysate; Lane2: Porcine Cerebellum lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Human MDM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MDM2. It has been selected for its ability to recognize MDM2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
  • Inactivation of the p53–KLF6–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
  • Sensitive and simultaneous surface plasmon resonance detection of free and p53-bound MDM2 proteins from human sarcomasPubmed:29637949

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