Polyclonal Antibody to Regenerating Islet Derived Protein 3 Gamma (REG3g)

REG-III; PAP1B; PAPIB; UNQ429; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 114.00 US$ 266.00 US$ 380.00 US$ 950.00 US$ 3,800.00
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Overview

Properties

Product No.PAE676Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryInfection immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE676Ra01-Recombinant Regenerating Islet Derived Protein 3 Gamma (REG3g)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Figure. DAB staining on IHC-P; Samples: Rat Lung Tissue.
DAB staining on IHC-P;
Samples: Rat Pancreas Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat REG3g Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat REG3g Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant REG3g, Rat.
Figure. Western Blot; Sample: Rat Intestine Tissue.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against REG3g. It has been selected for its ability to recognize REG3g in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:200-800
Immunohistochemistry: 5-20µg/mL;1:20-80
Immunocytochemistry: 5-20µg/mL;1:20-80
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Innate Stat3-mediated induction of the antimicrobial protein Reg3γ is required for host defense against MRSA pneumoniaPubMed: PMC3600913
Reciprocal Expression of Enteric Antimicrobial Proteins in Intestinal Graft-Versus-Host DiseasePubmed: 23927965
Hyperglycaemia inhibits REG3A expression to exacerbate TLR3-mediated skin inflammation in diabetes.pubmed:27830702
Enteric Delivery of Regenerating Family Member 3 alpha Alters the Intestinal Microbiota and Controls Inflammation in Mice With ColitisPubmed:29133078
Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infectionPubmed:29475562
Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock34840990