Polyclonal Antibody to Retinoic Acid Receptor Alpha (RARa)

RAR-A; NR1B1; RAR; RAR-Alpha; Nuclear Receptor Subfamily 1 Group B Member 1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 97.00 US$ 227.00 US$ 324.00 US$ 810.00 US$ 3,240.00
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Overview

Properties

Product No.PAA976Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Metabolic pathway Nutrition metabolism

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA976Mu01-Recombinant Retinoic Acid Receptor Alpha (RARa)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

IHC Images
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
AF488 staining on IF;
Sample: MCF7 cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse RARa Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant RARa, Mouse.
Figure. Western Blot; Sample: Lane1: Human A431 Cells; Lane2: Human HepG2 Cells.
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-mouse RARa Polyclonal Antibody (Catalog # PAA976Mu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against RARa. It has been selected for its ability to recognize RARa in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

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Citations

Retinoic acid receptor alpha is associated with tamoxifen resistance in breast cancerPubMed: PMC3759040
Retinoic acid receptor alpha: One of plasma biomarkers associated with exacerbation of chronic obstructive pulmonary diseasePubmed: 24111541