Recombinant Antibody to Interleukin 12B (IL12B) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

p40; IL12-B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit

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Overview
Properties
  • SourceRecombinant monoclonal antibody preparation, Host Mouse
  • Ig Isotype mouse IgG2b Kappa, Clone Number n/a
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Recombinant Antibody to Interleukin 12B (IL12B) Packages (Simulation)
  • Recombinant Antibody to Interleukin 12B (IL12B) Packages (Simulation)
  • Recombinant Antibody to Interleukin 12B (IL12B) After stimulated by IFNg(10ng/ml)plus LPS (100ng/ml) 20h,Human peripheral blood lymphocytes were fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human IL12B Recombinant Antibody (Catalog RAA058Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against IL12B. It has been selected for its ability to recognize IL12B in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Establishment and evaluation of a bead-based luminex assay allowing simultaneous quantification of equine IL-12 and IFN-γ.Pubmed: 23564769
  • Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress.pubmed:27384769
  • Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritisS1756464616303528
  • Photothermal-triggered Controlled Drug Release From Mesoporous Silica nanoparticles Based On Base-pairing Rules
  • Effects of Early Intervention with Antibiotics and Maternal Fecal Microbiota on Transcriptomic Profiling Ileal Mucusa in Neonatal PigsPubmed: 31963653
  • Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Pubmed: 32680553

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