Recombinant Antibody to Tau Protein (MAPT) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; Neurofibrillary tangle protein; Microtubule Associated Protein Tau; Paired helical filament-tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1

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Overview
Properties
  • SourceRecombinant monoclonal antibody preparation, Host Mouse
  • Ig Isotype mouse IgG2b Kappa, Clone Number n/a
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Recombinant Antibody to Tau Protein (MAPT) Packages (Simulation)
  • Recombinant Antibody to Tau Protein (MAPT) Packages (Simulation)
  • Recombinant Antibody to Tau Protein (MAPT) Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human MAPT Recombinant Antibody (Catalog RAB983Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against MAPT. It has been selected for its ability to recognize MAPT in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Effect of etanercept and lithium chloride on preventing secondary tissue damage in rats with experimental diffuse severe brain injuryPubmed:24452937
  • Wpływ omdlenia u młodocianych na stężenie białka tau w surowicy krwib09f7df8-1e7d-4665-a2a5-bf54dae0cfe9
  • Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
  • THE PRESENCE OF TAU PROTEIN IN BLOOD AS A POTENTIAL PROGNOSTIC FACTOR IN STROKE PATIENTSpubmed:28011949
  • Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
  • Differential hyperphosphorylation of tau-S199,-T231 and-S396 in organotypic brain slices of Alzheimer mice. A model to study early tau hyperphosphorylation using …Pubmed:29725295
  • The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in …

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