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ELISA Kit for Transferrin (TRF) Mus musculus (Mouse) Competition ELISA

TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin

  • Product No.CEC036Mu
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodCompetitive Inhibition
  • Assay Length2h
  • Detection Range12.35-1000ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 5.78ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Transferrin (TRF).No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 Ensure in stock; if not, free redo!
  • FOB US$ 279.00 US$ 398.00 US$ 1,791.00 US$ 3,383.00 US$ 27,860.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Transferrin (TRF) Packages (Simulation)
  • ELISA Kit for Transferrin (TRF) Packages (Simulation)
  • ELISA Kit for Transferrin (TRF) Results demonstration
  • CEC036Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Transferrin (TRF) and the recovery rates were calculated by comparing the measured value to the expected amount of Transferrin (TRF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-96 92
EDTA plasma(n=5) 83-97 87
heparin plasma(n=5) 80-99 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin (TRF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin (TRF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transferrin (TRF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 78-98% 82-103% 87-103%
EDTA plasma(n=5) 78-102% 82-95% 97-104% 84-91%
heparin plasma(n=5) 90-102% 90-98% 97-105% 83-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Transferrin (TRF)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Transferrin (TRF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Transferrin (TRF) and unlabeled Transferrin (TRF) (Standards or samples) with the pre-coated antibody specific to Transferrin (TRF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Transferrin (TRF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Transferrin (TRF) in the sample.

Reference

  • PLoS ONEIdentification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical SignificancePubMed: PMC3754973
  • Physiological researchThe Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Diseasepubmed:27782743

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