ELISA Kit for Neurogranin (NRGN) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Competition ELISA

Ng; NEUG; Protein Kinase C Substrate RC3

  • Product No.CEA404Mi
  • Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodCompetitive Inhibition
  • Assay Length2h
  • Detection Range39.06-10,000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 15.24pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Neurogranin (NRGN).No significant cross-reactivity or interference between Neurogranin (NRGN) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 In stock
  • FOB US$ 490.00 US$ 700.00 US$ 3,150.00 US$ 5,950.00 US$ 49,000.00
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  • ELISA Kit for Neurogranin (NRGN) Packages (Simulation)
  • ELISA Kit for Neurogranin (NRGN) Packages (Simulation)
  • ELISA Kit for Neurogranin (NRGN) Results demonstration
  • CEA404Mi.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Neurogranin (NRGN) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurogranin (NRGN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-104 93
EDTA plasma(n=5) 91-98 94
heparin plasma(n=5) 84-102 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurogranin (NRGN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurogranin (NRGN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurogranin (NRGN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 98-105% 91-105% 90-104%
EDTA plasma(n=5) 87-103% 95-103% 95-104% 86-97%
heparin plasma(n=5) 78-94% 82-99% 81-89% 80-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Neurogranin (NRGN)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurogranin (NRGN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurogranin (NRGN) and unlabeled Neurogranin (NRGN) (Standards or samples) with the pre-coated antibody specific to Neurogranin (NRGN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurogranin (NRGN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurogranin (NRGN) in the sample.

Citations

  • Alzheimer's & Dementia: Diagnosis, Assessment & Disease MonitoringPrediction of conversion from mild cognitive impairment to dementia with neuronally derived blood exosome protein profilePubmed:27408937
  • Annals of Clinical and Translational NeurologyPlasma neuronal exosomal levels of Alzheimer's disease biomarkers in normal agingPubmed:27231710
  • The FASEB JournalDecreased synaptic proteins in neuronal exosomes of frontotemporal dementia and Alzheimer’s diseasepubmed:27601437

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