ELISA Kit for Prostaglandin E2 (PGE2) Pan-species (General) Competition ELISA

PG-E2

  • Product No.CEA538Ge
  • Organism SpeciesPan-species (General) Same name, Different species.
  • Sample Typeserum, plasma and other biological fluids
  • Test MethodCompetitive Inhibition
  • Assay Length2h
  • Detection Range2.47-200pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.97pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Prostaglandin E2 (PGE2).No significant cross-reactivity or interference between Prostaglandin E2 (PGE2) and analogues was observed.
  • ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
  • DownloadInstruction Manual
  • UOM 48T96T 96T*5 96T*10 96T*100 Ensure in stock; if not, free redo!
  • FOB US$ 557.00 US$ 796.00 US$ 3,580.00 US$ 6,763.00 US$ 55,692.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit for Prostaglandin E2 (PGE2) Packages (Simulation)
  • ELISA Kit for Prostaglandin E2 (PGE2) Packages (Simulation)
  • ELISA Kit for Prostaglandin E2 (PGE2) Results demonstration
  • CEA538Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Prostaglandin E2 (PGE2) and the recovery rates were calculated by comparing the measured value to the expected amount of Prostaglandin E2 (PGE2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin E2 (PGE2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin E2 (PGE2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 78-104% 93-105% 78-96%
EDTA plasma(n=5) 78-101% 91-98% 95-103% 79-103%
heparin plasma(n=5) 91-101% 81-96% 79-96% 88-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Prostaglandin E2 (PGE2)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGE2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGE2 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGE2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGE2 in the sample.

Reference

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  • Journal of Gastroenterology and HepatologyCelecoxib attenuates hepatic cirrhosis through inhibition of epithelial-to-mesenchymal transition of hepatocytesPubmed:24909904
  • J Surg Res.Valproic acid protects septic mice from renal injury by reducing the inflammatory responsePubmed:24948542
  • Inflammation.?Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway.Pubmed:24962643
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  • PLOS ONEPenehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion InjuryPubMed: 26632817
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  • OncotargetMetformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axisPubmed:27058422
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  • OncotargetMetformin represses bladder cancer progression by inhibiting stem cell repopulation via COX2/PGE2/STAT3 axis.pubmed:27058422
  • Lipids.Dietary Arachidonic Acid Has a Time-Dependent Differential Impact on Adipogenesis Modulated via COX and LOX Pathways in Grass Carp Ctenopharyngodon idellus.pubmed:27747453
  • Fish Physiology and BiochemistryRole of cyclooxygenase-mediated metabolites in lipid metabolism and expression of some immune-related genes in juvenile grass carp (Ctenopharyngodon idellus) fed arachidonic acid.pubmed:28012026
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  • Food Funct.Flavonoids from sea buckthorn inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through the MAPK and NF-κB pathways.pubmed:28256654

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