Instant ELISA Kit for Dihydrotestosterone (DHT) 
5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone
- UOM
- FOB US$ 836.00 US$ 1,194.00 US$ 5,373.00 US$ 10,149.00 US$ 83,580.00
- Quantity
Overview
Properties
- Product No.IEA443Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - Downloadn/a
- CategoryEndocrinologyReproductive scienceHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Instant Dihydrotestosterone (DHT) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Dihydrotestosterone (DHT) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-103 | 101 |
| EDTA plasma(n=5) | 83-101 | 92 |
| heparin plasma(n=5) | 78-98 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Dihydrotestosterone (DHT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Dihydrotestosterone (DHT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Dihydrotestosterone (DHT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-103% | 79-93% | 95-105% | 80-101% |
| EDTA plasma(n=5) | 82-103% | 93-101% | 91-104% | 82-93% |
| heparin plasma(n=5) | 86-101% | 87-95% | 94-102% | 91-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 5 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Dihydrotestosterone (DHT). Standards or samples and HRP-labeled detection antibody specific to Instant Dihydrotestosterone (DHT) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Dihydrotestosterone (DHT), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Dihydrotestosterone (DHT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
Increment services
Citations
- Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar RatsIsrn: 130846
- Metabolic Changes and Hormonal Disturbances in Polycystic Ovarian Syndrome Rats and the Amelioration Effects of Metformin and/or Cinnamon ExtractionJofamericanscience: Source
- Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal modelPubmed:25256687
- Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2PubMed: 26088557
- Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARαpubmed:27413362
- Dihydrotestosterone Treatment Accelerates Autograft Reversal Sciatic Nerve Regeneration in RatsPubmed:29340845
- Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
- Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regressionPubmed: 30671954
- Plant cell culture extract of Cirsium eriophorum with skin pore refiner activity by modulating sebum production and inflammatory responsePubmed: 32816329
- Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast CancerPubmed:35323344
- Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cellsPubmed:35697131