Instant ELISA Kit for Thyroxine (T4) Pan-species (General) Instant ELISA

3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Instant ELISA Kit for Thyroxine (T4) Packages (Simulation)
  • Instant ELISA Kit for Thyroxine (T4) Packages (Simulation)
  • Instant ELISA Kit for Thyroxine (T4) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Instant Thyroxine (T4) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Thyroxine (T4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-98 90
EDTA plasma(n=5) 78-104 84
heparin plasma(n=5) 88-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Thyroxine (T4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Thyroxine (T4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Thyroxine (T4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-99% 85-93% 78-99% 80-103%
EDTA plasma(n=5) 78-90% 83-94% 89-102% 81-96%
heparin plasma(n=5) 80-101% 98-105% 88-104% 79-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 5 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 30 minutes at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 30 minutes at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Instant ELISA Kit for Thyroxine (T4)

Test principle

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Thyroxine (T4). Standards or samples and HRP-labeled detection antibody specific to Instant Thyroxine (T4) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Thyroxine (T4), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Thyroxine (T4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvaeScienceDirect: S0166445X12000215
  • Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axisPubMed: 23220413
  • Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studiesLifesciencesite: Source
  • Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay.ScienceDirect: S0166445X13002129
  • Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvaePubmed:25456233
  • Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarumscience:S0045653516302016
  • Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Micepubmed:28119573
  • Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspringpubmed:27803384
  • Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.pubmed:28322267
  • Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus).pubmed:28187359
  • Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disordershandle:123456789
  • Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes09291016.2016.1275393
  • Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reductionpubmed:28973666
  • Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead10.1016/j.envpol.2017.06.053
  • 四溴双酚A对菲律宾蛤仔的生长抑制效应机理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum5790267088906950000
  • Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats10.4314/tjpr.v16i5.9
  • Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult10371/137708
  • Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish.pubmed:28624628
  • Stereoselective degradation and thyroid endocrine disruption of lambda-cyhalothrin in lizards (Eremias argus) following oral exposurepubmed:28970022
  • Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafishPubmed:29995391
  • Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
  • Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons10.1093:toxsci
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liverPubmed:29555828
  • Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult PatientPubmed:29593791
  • Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes10.5194:aab-61-191-2018
  • Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspringPubmed: 30243188
  • Metabolism of diflubenzuron in lizard (Eremias argus) and comparative toxicity of diflubenzuron and its metabolitePubmed: 30346759
  • Elastographic and morphological testicular changes in hypothyroidism–an experimental studyDoi: 10.2478/jvetres-2018-0049
  • Dose-dependent effects of flufenoxuron on thyroid system of mature female lizards (Eremias argus) and their offspringPubmed: 30448662
  • Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
  • Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing …Pubmed: 30518657
  • Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafishDoi: 10.1016/j.scitotenv.2019.02.198
  • Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPAPubmed: 30844590
  • Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in ratsDoi: 10.2478/jvetres-2019-0008
  • The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice
  • Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevisPubmed: 31276910
  • Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvaePubmed: 31539807
  • Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
  • Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balancePubmed: 31793369
  • Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryosPubmed: 32172180
  • The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult micePubmed: 31982645
  • Effect of triadimefon and its metabolite on adult amphibians Xenopus laevisPubmed: 31743868
  • Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axisPubmed: 33177714
  • Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio)Pubmed: 32525014
  • Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlorPubmed: 32468518
  • Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathyPubmed: 32442899
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa …
  • Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio)
  • Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation33433934
  • High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression33758937
  • Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism34019632
  • Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development34126306
  • Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism34092241
  • Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect33647619
  • The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver34683936
  • Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations34933138
  • Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvaePubmed:35131581
  • Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio)Pubmed:35448459
  • Fluorene-9-Bisphenol Induced Thyroid-Disrupting Effects and Histopathological Alterations in Adult Zebrafish (Danio Rerio)

Recommend products