Multiplex Assay Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) Bos taurus; Bovine (Cattle) Multiplex ELISA

B-Cell CLL/Lymphoma 2

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LMA778Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-96 84
EDTA plasma(n=5) 81-101 93
heparin plasma(n=5) 93-101 97
sodium citrate plasma(n=5) 80-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 86-103% 79-102% 85-92%
EDTA plasma(n=5) 97-105% 91-103% 80-97% 80-102%
heparin plasma(n=5) 83-91% 96-104% 78-99% 85-92%
sodium citrate plasma(n=5) 86-101% 94-105% 86-101% 83-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:Bcl2) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for B-Cell Leukemia/Lymphoma 2 (Bcl2) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • Analysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Neurotoxic effect of lead on rats: Relationship to ApoptosisNCBI: PMC3883608
  • Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosinPubmed: 24327573
  • Vitamin D ameliorates hepatic ischemic/reperfusion injury in ratsPubmed:24752458
  • Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activitiesPubmed:25289771
  • Serum Bcl-2 Values in Children with Pulmonary HypertensionPubmed:25381623
  • Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisPubmed:25511416
  • Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-γ, NF-κB and Nrf2/HO-1 Signaling PathwaysPubMed: 26196679
  • Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the RatPubMed: 26700436
  • Hepatoprotective Effects of Rubus idaeus L. Leaves against CCl4-Induced Liver Injury via Antioxidant, Anti-inflammatory and Antiapoptotic Mechanismsopenview:626440
  • THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATSpublication:298838934
  • Neuroprotective Effect of Nanodiamond in Alzheimer's Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 SignalingPubmed:26897372
  • The ameliorative potential of ethanolic extract of propolis on hematotoxicity and structural neuronal damage in hyperthermia-exposed ratspubmed:27746870
  • In Vivo Characterization of ARN14140, a Memantine/Galantamine-Based Multi-Target Compound for Alzheimer's Diseasepubmed:27609215
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction bydanhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms.pubmed:27729285
  • Mangiferin protects against‭ intestinal ischemia/reperfusion-induced‭ liver injury:‬‬ Involvement of PPAR-‭ γ, GSK-3β and Wnt/β-catenin pathway‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬pubmed:28506911
  • Comparative Effects of Stevia rebaudiana and Aspartame on hepato-renal function of diabetic rats: Biochemical and Histological Approaches59b5141c458515a5b49377cf
  • A Novel Role of SIRT1/ FGF-21 in Taurine Protection Against Cafeteria Diet-Induced Steatohepatitis in Ratspubmed:28942443
  • Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in ratspubmed:28554128
  • Distinctive expression signatures of serum microRNAs in ischaemic stroke and transient ischaemic attack patientspubmed:28251236
  • A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in ratspubmed:28709897
  • Neuroprotective Effect of Nanodiamond in Alzheimer’s Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signalingpubmed:26897372
  • Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin10.1007/s13530-017-0325-1
  • Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heartpubmed:28686977
  • Association of Bax and Bcl-2 Functional Polymorphisms and Protein Levels with Posttraumatic Stress DisorderpaperID:82598
  • Structural optimization and additional targets identification of antisense oligonucleotide G3139 encapsulated in a neutral cytidinyl-lipid combined with a cationic lipid …Pubmed: 30660994
  • Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathwayPubmed: 30723634
  • INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA
  • Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptorsPubmed: 31048034
  • Metallothionein-1 is associated with osteoarthritis disease activity and suppresses proinflammatory cytokines production in synovial cellsPubmed: 31465913
  • LncRNA SNHG3 promotes clear cell renal cell carcinoma proliferation and migration by upregulating TOP2APubmed: 31505165
  • LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating …Pubmed: 32197949
  • Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cellsPubmed: 33206337
  • Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of …Pubmed: 32915453
  • Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascadePubmed: 32869669
  • Potential anti-tumor activity of kefir-induced juglone and resveratrol fractions against Ehrlich ascites carcinoma-bearing BALB/c mice
  • Curcumin loaded nanostructure hybrid lipid capsules for co-eradication of breast cancer and cancer stem cells with enhanced anticancer efficacy
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • The Effect of Sodium Metabisulphite on Apoptosis in the Experimental Model of Parkinson's Disease
  • Cilostazol mitigates mesenteric ischemia/reperfusion-induced lung lesion: Contribution of PPAR-¦Ã, NF-¦ÊB, and STAT3 crosstalk33310046
  • Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ¡­33491863
  • Magnesium Isoglycyrrhizinate Alleviates Arsenic Trioxide-Induced Cardiotoxicity: Contribution of Nrf2 and TLR4/NF-¦ÊB Signaling Pathway33603344
  • Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent)
  • Expression of Apoptotic Markers Bcl-2 and Bax in the Vascular Wall
  • [8]‐Gingerol exerts anti‐myocardial ischemic effects in rats via modulation of the MAPK signaling pathway and L‐type Ca2+ channels34390539
  • Lycopene attenuates zearalenone-induced oxidative damage of piglet sertoli cells through the nuclear factor erythroid-2 related factor signaling pathway34482067
  • Allicin ameliorates renal ischemia/reperfusion injury via inhibition of oxidative stress and inflammation in rats34426252
  • Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity34729716
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026
  • Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn.Pubmed:35398498
  • Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and …Pubmed:35640776
  • Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosisPubmed:35636581
  • Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino …Pubmed:35624893
  • Nephroprotective effect of magnesium isoglycyrrhizinate against arsenic trioxide‑induced acute kidney damage in micePubmed:35317438
  • Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats

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