Multiplex Assay Kit for Corticosterone (Cort) ,etc. by FLIA (Flow Luminescence Immunoassay) 
Corticosrone
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 497.00 US$ 516.00 US$ 544.00 US$ 583.00 US$ 621.00 US$ 678.00 US$ 764.00 US$ 955.00
- Quantity
Overview
Properties
- Product No.LMA540Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityEndocrinologyHormone metabolism
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Corticosterone (Cort) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Corticosterone (Cort) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 90-101 | 97 |
| EDTA plasma(n=5) | 91-101 | 97 |
| heparin plasma(n=5) | 80-97 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticosterone (Cort) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticosterone (Cort) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticosterone (Cort) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-97% | 99-105% | 79-97% | 96-104% |
| EDTA plasma(n=5) | 82-95% | 86-94% | 98-105% | 93-102% |
| heparin plasma(n=5) | 95-102% | 79-95% | 97-104% | 78-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:Cort) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
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- Effects of Gypenosides on Acute Stress in MicePapersearch: Source
- Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stressPubmed:25911494
- Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in MiceCom:Source
- Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized RatsPubmed:24366191
- Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male ratsPubmed:24440345
- Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantationPubmed:25238024
- Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic ratsPubMed: 26379948
- Trivalent Chromium Attenuated Corticosterone Secretion and Actions in Adrenocorticotropic Hormone-Stimulated RatsIn_Press: 15-058.Pdf
- Ameliorating effects of gypenosides on chronic stress-induced anxiety disorders in micePubMed: 26370834
- Rats with differential self-grooming expression in the elevated plus-maze do not differ in anxiety-related behaviorsPubMed: 26142783
- Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosteronePubmed:26855320
- Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorderPubmed:26934364
- Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depressionPubmed:26773866
- Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression.Pubmed:27212922
- Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathwayPubmed:27102619
- Apelin-13 exerts antidepressant-like and recognition memory improving activities in stressed ratsPubmed:26853763
- Dopamine D2-like receptors modulate freezing response, but not the activation of HPA axis, during the expression of conditioned fear.pubmed:27766352
- Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus.pubmed:28161195
- Effects of (-)-Sesamin on Chronic Stress-Induced Anxiety Disorders in Micepubmed:27995494
- Role of serotonin in the intestinal mucosal epithelium barrier in weaning mice undergoing stress-induced diarrheapubmed:29260435
- The Role of Psychological Stress on Heart Autophagy in Mice With Heart Failurepubmed:28691995
- Environmental perturbation of the circadian clock during pregnancy leads to transgenerational mood disorder-like behaviors in micepubmed:28974783
- Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning micepubmed:28633646
- Anxiety-like but not despair-like behaviors are further aggravated by chronic mild stress in the early stages of APPswe/PS1dE9 transgenic micedoi.org/10.1101/202283
- Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis.pubmed:28959186
- Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female ratsPubmed:30028991
- Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons10.1093:toxsci
- Multigenerational effects of dietary macronutrients intake on the metabolic phenotype of male Wistar rats10.1016:j.nut.2018.06.008
- Depressive-and anxiety-like phenotypes in young adult APPSwe/PS1dE9 transgenic mice with insensitivity to chronic mild stressPubmed:30012417
- Blockade of AT1 type receptors for angiotensin II prevents cardiac microvascular fibrosis induced by chronic stress in Sprague–Dawley ratsPubmed:29676198
- Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female MalePubmed:29737476
- Curcumin attenuates chronic intermittent hypoxia-induced brain injuries by inhibiting AQP4 and p38 MAPK pathwayPubmed:29758366
- Chronic treatment with dexamethasone alters clock gene expression and melatonin synthesis in rat pineal gland at nightPubmed:30046256
- Tannins from fruits attenuates GABA antagonist‐induced anxiety‐like behaviour modulation of neurotransmittersPubmed: 30198561
- Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
- HMGB1-Mediated Differential Response on Hippocampal Neurotransmitter Disorder and Neuroinflammation in Adolescent Male and Female Mice following Cold ExposurePubmed: 30476565
- Establishment and evaluation of a novel mouse model of peri/postmenopausal depressionDoi: 10.1016/j.heliyon.2019.e01195
- Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome compositionPubmed: 31015500
- Antidepressant-like effects of dietary gardenia blue pigment derived from genipin and tyrosineDoi: 10.1039/C9FO00480G
- Synaptic structure and alterations in the hippocampus in neonatal rats exposed to lipopolysaccharidePubmed: 31288048
- Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in micePubmed: 31431066
- MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression ModelPubmed: 31540304
- Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose …Pubmed: 32249244
- GABA-mediated activated microglia induce neuroinflammation in the hippocampus of mice following cold exposure through the NLRP3 inflammasome and …Pubmed: 33166810
- Anxiolytic and Anti‑depressive Like Effects of Translocator Protein (18 kDa) Ligand YL‑IPA08 in a Rat Model of Postpartum DepressionPubmed: 32383026
- Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
- Neurobiological determinants of depressive-like symptoms in rodents
- Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax …
- Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice33294147
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- O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993
- Sleep disturbance induces depressive behaviors and neuroinflammation by altering the circadian oscillations of clock genes in rats33785408
- A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …Pubmed:35281603
- Monochromatic blue light not green light exposure is associated with continuous light-induced hepatic steatosis in high fat diet fed-mice via oxidative stressPubmed:35588616