Multiplex Assay Kit for Dopamine (DA) ,etc. by FLIA (Flow Luminescence Immunoassay) 
2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 497.00 US$ 516.00 US$ 544.00 US$ 583.00 US$ 621.00 US$ 678.00 US$ 764.00 US$ 955.00
- Quantity
Overview
Properties
- Product No.LMA851Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionCardiovascular biologyReproductive scienceNeuro science
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Dopamine (DA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Dopamine (DA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 91-101 | 95 |
| EDTA plasma(n=5) | 88-96 | 91 |
| heparin plasma(n=5) | 78-104 | 96 |
| sodium citrate plasma(n=5) | 89-97 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine (DA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine (DA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dopamine (DA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-101% | 79-95% | 80-96% | 80-95% |
| EDTA plasma(n=5) | 78-98% | 97-105% | 79-88% | 87-94% |
| heparin plasma(n=5) | 88-102% | 86-102% | 97-104% | 87-101% |
| sodium citrate plasma(n=5) | 94-102% | 87-97% | 91-98% | 88-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:DA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis PatientsPubmed: 23530612
- Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial PerturbationsPubmed:25502462
- Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological AberrationsPubmed:25526861
- Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adultsPubmed:25824645
- Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activityPubMed: 25843409
- Catha edulis chewing effects on treatment of paranoid schizophrenic patientsPubMed: 25926735
- Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathwaysPubMed: 25752913
- Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair testscience:S2314853516300166
- Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in ratspubmed:27365173
- Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes.pubmed:27488111
- Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in ratsS0899900716302350
- Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats pubmed:28241996
- Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Ratsarticle_1486_c0fa9f8afe5f70b407e7195bcfd55c70
- High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in ratsPubmed:29842916
- Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor …Pubmed:29854627
- Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytesPubmed:28948423
- Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll …Pubmed:29410018
- Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring ratsPubmed: 30423388
- Dopamine levels in human tear fluidPubmed: 30574889
- Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles
- Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brainPubmed: 31134480
- Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome
- Neurotoxicity of Mn3O4 nanoparticles: Apoptosis and dopaminergic neurons damage pathwayPubmed: 31740235
- Polydatin protects SH-SY5Y in models of Parkinson's disease by promoting Atg5-mediated but parkin-independent autophagyPubmed: 31926197
- Iron overload induced by IRP2 gene knockout aggravates symptoms of Parkinson's diseasePubmed: 31904393
- Neurotransmitter system aberrations in patients with drug addictionPubmed: 32804296
- Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemiaPubmed: 32517478
- Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine
- Neuroprotective effects of pulicaria undulata essential oil in rotenone model of parkinson's disease in rats: Insights into its anti-inflammatory and anti-oxidant effects
- The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice33303990
- Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6?¡33433498
- Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡33762821
- O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993
- A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster34654742
- Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson's Disease34197084
- Effects of early life stress during stress hyporesponsive period (SHRP) on anxiety and curiosity in adolescent ratsPubmed:35141770