Multiplex Assay Kit for Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) 
Histamin
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 497.00 US$ 516.00 US$ 544.00 US$ 583.00 US$ 621.00 US$ 678.00 US$ 764.00 US$ 955.00
- Quantity
Overview
Properties
- Product No.LMA927Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategorySignal transductionInfection immunity
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-95 | 89 |
| EDTA plasma(n=5) | 94-101 | 97 |
| heparin plasma(n=5) | 90-97 | 94 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Histamine (HA) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-104% | 93-102% | 82-95% | 81-103% |
| EDTA plasma(n=5) | 80-101% | 97-105% | 89-97% | 79-94% |
| heparin plasma(n=5) | 79-89% | 81-101% | 80-105% | 85-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:HA) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in micePubmed: 21981059
- Comparison of sensitization between β-lactoglobulin and its hydrolysatesDigital: 875
- Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injectionPubMed: 26372445
- Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrheaPubmed:26940641
- Induction of immune responses and allergic reactions in piglets by injecting glycinindoi:1828051X.2016.1144488
- Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanismfiles:ijcep0019305.pdf
- Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbuminpmc:PMC4734762
- Evaluation of anaphylactoid constituents in vitro and in vivo.pubmed:27984711
- Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection propertiesDOI: 10.1111/jocd.12349
- Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat ModelPubmed: 30781605
- Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats
- MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL
- Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trialPubmed: 32030780
- Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injectionPubmed: 32302806
- Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in ratPubmed: 32395767
- A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapyPubmed: 32332752
- CCR3‑shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cellsPubmed: 32742345
- Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of …Pubmed: 32892931
- Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq
- Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs34014033