Multiplex Assay Kit for Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay) 
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 427.00 US$ 443.00 US$ 468.00 US$ 501.00 US$ 534.00 US$ 583.00 US$ 657.00 US$ 821.00
- Quantity
Overview
Properties
- Product No.LMA242Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-96 | 89 |
| EDTA plasma(n=5) | 92-105 | 95 |
| heparin plasma(n=5) | 79-90 | 83 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-103% | 83-97% | 78-97% | 87-94% |
| EDTA plasma(n=5) | 95-105% | 96-104% | 93-101% | 94-104% |
| heparin plasma(n=5) | 80-101% | 91-104% | 91-103% | 96-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:NES1) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards,Labeled antigen and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest.A competitive inhibition reaction is launched between biotin labeled analytes of interest and unlabeled analytes of interest (Standards or samples) with the pre-coated antibody specific to analytes of interest. Following a wash to remove any unbound substances, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE) is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. The MFI developed is reverse proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic DisorderPsikofarmakoloji: 2043
- High plasma nesfatin-1 level in patients with major depressive disorderPubMed: 21163317
- Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitusSpringerLink: h978088657555w16
- Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder Psikofarmakoloji: 2212
- Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorderPubMed: 22595767
- Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female RatsIjvst: Source
- Decreased serum nesfatin-1 levels in endometriosisPubmed: 24702902
- Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder.Psikofarmakoloji: Source
- Role of NUCB2/nesfatin-1 as a possible biomarker.Pubmed: 23537082
- A role of nesfatin-1/NucB2 in dehydration-induced anorexiaPubmed:24829503
- Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndromePubmed:24920281
- The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study.Pubmed:25448651
- The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markersPubmed:25581765
- New hormones to predict the severity of gallstone-induced acute pancreatitisPubmed:25599787
- Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?PubMed: 26088934
- Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished ChildrenPubMed: 26831553
- Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndromePubMed: 26295295
- Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1Sayilar: 294
- Nesfatin-1 levels and metabolic markers in polycystic ovary syndromePubMed: 26062107
- Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage*Pubmed:26872406
- Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairmentP.1.e.012.pdf
- Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage.pubmed:26872406
- Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Diseasepubmed:28137218
- Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetespubmed:28977160
- Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving?pubmed:28529367
- Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγPubmed:29604590
- Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodelingPubmed:29627363
- Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patientsPubmed:29615007
- Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad ShidfarPubmed: 30232744
- An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages
- Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levelsPubmed: 32311618
- Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with ObesityPubmed: 33027789
- Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke33283259
- Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder