Multiplex Assay Kit for Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Mus musculus (Mouse) Multiplex ELISA

SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

(Note: Up to 8-plex in one testing reaction)

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  • Multiplex Assay Kit for Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Packages (Simulation)
  • Multiplex Assay Kit for Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) Results demonstration
  • LME912Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-99 93
EDTA plasma(n=5) 90-98 94
heparin plasma(n=5) 80-88 85
sodium citrate plasma(n=5) 79-104 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 86-95% 89-99% 95-103%
EDTA plasma(n=5) 81-99% 93-101% 95-102% 81-91%
heparin plasma(n=5) 80-93% 92-102% 93-101% 82-91%
sodium citrate plasma(n=5) 97-105% 89-104% 81-97% 79-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SIRT1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

Multiplex Assay Kit for Sirtuin 1 (SIRT1) ,etc. by FLIA (Flow Luminescence Immunoassay)

Test principle

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.

Giveaways

Citations

  • SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular DiseasesPlos: Source
  • A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in RatsPubmed:25501750
  • Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive ImpairmentPubmed:25589731
  • Increases in peripheral SIRT1: A new biological characteristic of asthmaPubMed: 26040995
  • A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly:PubMed: 25785999
  • Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease.pubmed:27522360
  • The potential curative effect of rebamipide in hepatic ischemia/reperfusion injurypubmed:28361247
  • A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery diseasepubmed:28043667
  • Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trialpubmed:28029409
  • Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han populationpubmed:29189974
  • Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1)pubmed:28974454
  • Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunctionpubmed:28919305
  • Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …Pubmed:30037068
  • Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementiaPubmed:30071285
  • The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic NephropathyPubmed: 30504713
  • Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical studyPubmed: 30556451
  • Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic …
  • Influência do tratamento da doença periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doença arterial coronária
  • Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility
  • Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
  • Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of …Pubmed: 30956976
  • Vasohibin-1 in T2DM with different UACR
  • The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance HemodialysisPubmed: 32214805
  • Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factorPubmed: 33045669
  • Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте
  • Dietary buckwheat enhances sirtuin1 without calorie restriction
  • Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study33447019
  • The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡­33396247
  • Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease33864594
  • Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ¡­34052296
  • Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories34174262
  • Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats34763933
  • Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease34774622
  • Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient RatsPubmed:35163435
  • Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter?Pubmed:35683374
  • OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress

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