Multiplex Assay Kit for Tryptase (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) 
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
(Note: Up to 8-plex in one testing reaction)
- UOM
- FOB US$ 450.00 US$ 468.00 US$ 494.00 US$ 528.00 US$ 563.00 US$ 615.00 US$ 693.00 US$ 866.00
- Quantity
Overview
Properties
- Product No.LMB070Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsFLIA Kit for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseInfection immunityRheumatology
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Tryptase (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Tryptase (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-105 | 102 |
| EDTA plasma(n=5) | 86-98 | 92 |
| heparin plasma(n=5) | 87-94 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptase (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptase (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tryptase (TPS) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-98% | 79-90% | 97-104% | 79-94% |
| EDTA plasma(n=5) | 93-105% | 90-104% | 85-92% | 88-95% |
| heparin plasma(n=5) | 94-103% | 97-104% | 99-105% | 96-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:TPS) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed is proportional to the concentration of analytes of interest in the sample.
Giveaways
Increment services
Citations
- Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polypsPubMed: 22534535
- Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2Pubmed:24530123
- Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis HerpetiformisPubmed:25400334
- A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in RatsPubmed:25541997
- Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgeryPubmed:25677271
- Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary ReportPubMed: 26089601
- Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitisPubMed: 26049029
- Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell originsJournals: Mi
- Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell OriginsPubmed:27057098
- An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
- Dengue virus-elicited tryptase induces endothelial permeability and shockPubmed: 31265436
- Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm FunctionPubmed: 31998139
- Biomarkers of Allergic Asthma and their Association with Serum Parameters
- Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
- Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive …34650557
- Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia SyndromePubmed:35612327