17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14)

17-beta-hydroxysteroid dehydrogenases, such as HSD17B14, are primarily involved in metabolism of steroids at the C17 position and also of other substrates, such as fatty acids, prostaglandins, and xenobiotics.

The deduced 270-amino acid protein has a calculated molecular mass of 28.0 kD. It has all the characteristics of SDRs, including a cofactor-binding Rossman-fold domain and highly conserved motifs in the nucleotide-binding site and active site. Northern blot analysis detected HSD17B14 expression in liver, kidney, and retina.recombinant human DHRS10 converted NAD+ to NADH in the presence of estradiol, testosterone, or 5-androstene-3-beta,17-beta diol. Nonsaturable kinetics were found for testosterone, and no conversion was observed in the presence of NADPH.