Abhydrolase Domain Containing Protein 10 (ABHD10)

ABHD10 belongs to the AB hydrolase superfamily. In contrast, none of the other 27 SH activities identified in the brain membrane proteome were significantly altered by 70, with the exception of ABHD10, which showed a modest (30%) but significant reduction in activity in 70-treated proteomes.12 Follow-up studies, however, failed to reveal any inhibition of recombinant ABHD10 by 70 at concentrations up to 100 íM, indicating that this enzyme is unlikely to be a true target of 70. Collectively, these data indicate that 70 is a highly selective inhibitor of ABHD6 compared to other brain SHs. From a technical perspective, the results also highlight the superior resolution afforded by LC-MS compared to gel-based methods for broadly assessing the protein targets of chemical probes.