Acyl Coenzyme A Thioesterase 2 (ACOT2)

Acyl-CoA thioesterases, such as ACOT2, are a group of enzymes that hydrolyze CoA esters, such as acyl-CoAs, bile CoAs, and CoA esters of prostaglandins, to the corresponding free acid and CoA.

The corresponding gene, which the authors called PTE2, encodes a deduced 421-amino acid protein with a predicted molecular mass of 46.3 kD. PTE2 shares 76% and 72.3% sequence identity with rat CTE1 and rat MTE1, respectively, and only 19% with human PTE1. Immunofluorescence assays showed that Myc-tagged PTE2 in human fibroblasts was imported into peroxisomes and targeted to the peroxisome lumen, and that PTE2 localizes to the cytosol of a Zellweger syndrome skin fibroblast cell line that is unable to import peroxisomal matrix proteins.