Adenosine Deaminase Domain Containing Protein 2 (ADAD2)

Adenosine Deaminase Domain Containing 2 expression: blood; brain; eye; placenta; spleen; testis; thyroid; leukemia; normal; fetus; infant; juvenile; adult.

Adenosine deaminase is an enzyme (EC 3.5.4.4) involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues.Total plasma ADA can be measured using high performance liquid chromatography, enzymatic or colorimetric techniques. Perhaps the simplest system is the measurement of the ammonia released from adenosine when broken down to inosine. After incubation of plasma with a buffered solution of adenosine the ammonia is reacted with a Berthelot reagent to form a blue colour which is proportionate to the amount of enzyme activity.