Ankyrin Repeat And SOCS Box Containing Protein 15 (ASB15)

ASB15 has a role in early myoblast differentiation and that its effects may be mediated in part by the PI3K/Akt signal transduction pathway. Overexpression of ASB15 delays differentiation and alters protein turnover in mouse C2C12 myoblasts. However, the extent of ASB15 regulation of differentiation and molecular pathways underlying this activity are unknown. ASB15 controls myoblast differentiation through its regulation of these kinases. Stably transfected myoblasts overexpressing ASB15 (ASB15+) demonstrated decreased differentiation, whereas attenuation of ASB15 expression (ASB15-) increased differentiation. However, ASB15+ cells had less abundance of the phosphorylated mitogen-activated protein kinase (active) form, despite decreased differentiation relative to control myoblasts (ASB15Con).