Arsenite Inducible RNA Associated Protein Like Protein (AIRAPL)

The C-terminal domain of Airapl also has 3 p38 MAP kinase (MAPK14) phosphorylation sites. Airapl and Airap (ZFAND2A) share 60% identity in their N-terminal ends, but their C termini differ. EST database analysis detected broad AIRAPL expression in human and mouse tissues. Immunohistochemical analysis showed that mouse Airapl was expressed in the endoplasmic reticulum of transfected COS-1 cells, and this localization required the C-terminal prenylation motif.Cytosolic Airap associated with proteasomes several hours after Airapl. Binding of Airap and Airapl to proteasomes enhanced the ability of proteasomes to digest a small peptide substrate. Domain swapping between Airap and Airapl revealed that the C-terminal domain of Airapl directed arsenite-regulated proteasome complex formation.