Axin Interactor, Dorsalization Associated (AIDA)

Rui et al. (2007) cloned mouse and zebrafish Aida and identified the Xenopus and human orthologs by database analysis. The deduced human and mouse proteins contain 306 and 305 amino acids, respectively, and all orthologs share a high degree of identity. Northern blot analysis detected a 2.5-kb AIDA transcript in all human tissues examined.

Using yeast 2-hybrid analysis, immunoprecipitation analysis, and protein pull-down assays, Rui et al. (2007) showed that vertebrate Aida interacted with Axin (AXIN1). Aida inhibited Axin-mediated JNK activation by disrupting Axin homodimerization. In zebrafish embryos, Aida overexpression induced ventralization and inhibited axin-induced dorsalization.Hartz (2008) mapped the AIDA gene to chromosome 1q41 based on an alignment of the AIDA sequence with the genomic sequence (build 36.1).