BMS1 Homolog (BMS1)

Bms1 is required for viability in yeast. Using conditional mutant alleles of Bms1, they observed that depletion of Bms1 caused defects in cleavage and processing of yeast 35S pre-rRNA and the disappearance of 40S ribosomal subunits. Glycerol gradient analysis of Bms1 mutants and Rcl1 showed that Bms1 and Rcl1 cosediment together in the 10S complex and associate with nascent ribosomes. Immunoprecipitation studies showed that Bms1 associates with U3 snoRNA. Mutations of BMS1 amino acids implicated in GTP/GDP binding affected BMS1 activity in vivo.The deduced protein contains 1,285 amino acids. Northern blot analysis detected expression in all tissues examined.The deduced 1,183-amino acid yeast protein has a calculated molecular mass of 135.5 kD.