BUD31 Homolog (BUD31)

The Xenopus G10 mRNA is a maternal transcript that is translationally activated during oocyte maturation. Using a PCR-subtractive hybridization strategy, Hla et al. (1995) isolated cDNAs that were induced by phorbol myristic acetate in human umbilical vein endothelial cells.

One cDNA, designated EDG2, encoded a predicted 144-amino acid protein with 93% sequence identity to the predicted G10 protein. Both proteins contained an N-terminal acidic domain and a cysteine-rich C-terminal domain containing a potential C2C2-type zinc finger motif. The in vitro translated human EDG2 protein had a molecular mass of approximately 18 kD. RT-PCR analysis indicated that EDG2 is expressed ubiquitously.