Anterior Gradient Protein 3 (AGR3)

Fluorescence-tagged BCMP11 localized in secretory or endosome-like organelles in transfected breast carcinoma cell lines. Immunohistochemical analysis showed that expression of BCMP11 was restricted in normal tissues, with significant staining of only the epithelial lining of colonic mucosa. However, BCMP11 was detected in 43 of 58 (74%) breast cancer tissues tested, but not in normal breast ductal epithelial cells. In cancerous epithelial cells, BCMP11 localized to the cytoplasm.Expression of AG2 and AG3 showed a positive correlation with expression of estrogen receptor and a negative correlation with expression of EGF receptor (EGFR). Yeast 2-hybrid analysis identified C4.4A and extracellular alpha-dystroglycan (DAG1) as binding partners for both AG2 and AG3.