CCR4 NOT Transcription Complex Subunit 2 (CNOT2)

NOT2 protein shares 26% overall sequence identity with the 191-amino acid yeast NOT2 protein. Compared to yeast NOT2, human NOT2 has an N-terminal extension. Human NOT2 contains an N-terminal putative bipartite nuclear localization signal. recombinant human NOT2 can interact with yeast NOT1 and an N-terminally truncated human NOT1 protein; it did not significantly interact with human NOT3 or yeast CCR4. Northern blot analysis detected a 3.2-kb NOT2 transcript in all human tissues examined, namely brain, heart, lung, liver, kidney, small intestine, colon, spleen, thymus, peripheral blood leukocytes, skeletal muscle, and placenta. Like the expression of human NOT1, NOT3, and CALIF, NOT2 expression was high in brain, kidney, and placenta and very low in skeletal muscle and colon.