CXXC Finger Protein 1 (CXXC1)

Proteins that contain a CXXC motif within their DNA-binding domain, such as CXXC1, recognize CpG sequences and regulate gene expression. The deduced 656-amino acid protein has a calculated molecular mass of 76 kD. CGBP contains an N-terminal PHD domain, followed by a CXXC domain, a central basic region, a coiled-coil domain, and an imperfect PHD domain near its C terminus. Northern blot analysis detected a 2.6-kb CGBP transcript in all tissues examined. Western blot analysis of nuclear extracts of a myeloid cell line detected endogenous CGBP at an apparent molecular mass of 88 kD.CGBP failed to bind oligonucleotides in which the CpG motif was mutated or methylated, and it did not bind single-stranded DNA or RNA probes.