Carbohydrate Sulfotransferase 14 (CHST14)

D4ST1 catalyzes the 4-O-sulfation of N-acetylgalactosamine (GalNAc) residues in dermatan sulfate, a stereoisomeric form of chondroitin sulfate that contains varying proportions of iduronic acid in place of glucuronic acid .

The deduced 376-amino acid protein has a calculated molecular mass of 43 kD. D4ST1 is a type II membrane protein with an N-terminal transmembrane region, binding sites for 3-prime-phosphoadenosine-5-prime-phosphosulfonate (PAPS), and 2 potential N-glycosylation sites. D4ST1 shares significant similarity with other sulfotransferases. The N-terminal regions are most variable. Northern blot analysis detected a 2.4-kb transcript in heart, placenta, liver, and pancreas, with significantly lower levels in skeletal muscle and kidney.